主讲课程

分子生物学、分子生物学实验、分子酶学与生物催化

个人简介

李艳，博士，教授，硕士生导师。1998年毕业于合肥工业大学环境工程专业，获工学学士学位。2008年毕业于南京工业大学生物化工专业，获工学博士学位。2006—2009年先后三次前往德国斯图加特大学生物化学技术研究所和生物工程研究所进行联合培养和项目合作。2008年至今在南京工业大学生物与制药工程学院从事教学科研工作。研究领域涉及酶工程、天然产物生物催化转化等。近年来主要在甜菊糖苷系列产品和其他高附加值糖苷类化合物的生物合成方面开展相关研究。目前在研课题包括国家重点研发计划项目子任务2项和企业委托项目2项。已发表SCI收录论文40篇，申请国家发明专利20余项，其中授权10余项。

代表性成果

1. Pan Huayi, Xiao Liang, Tang Kexin, Xia Haojun, Li Yan*, Jia Honghua*, Wei Ping, Yan Ming. Screening UDP-glycosyltransferases for effectively transforming Stevia glycosides: enzymatic synthesis of glucosylated derivatives of rubusoside. Journal of Agricultural and Food Chemistry, 2022, 70: 15178–15188

2. Yu Jie, Tao Yehui, Pan Huayi, Lin Lei, Sun Jinyuan, Ma Ruiqi, Li Yan*, Jia Honghua*. Mutation of Stevia glycosyltransferase UGT76G1 for efficient biotransformation of rebaudioside E into rebaudioside M. Journal of Functional Foods. 2022, 92: 105033

3. Lei Jiping, Tang Kexin, Zhang Ting, Li Yan*, Gao Zhen, Jia Honghua*. Efficient production of 2‑O‑α‑D‑glucosyl glycerol catalyzed by an engineered sucrose phosphorylase from Bifidobacterium longum. Applied Biochemistry and Biotechnology, 2022, 194: 5274–5291

4. Chen Liangliang, Pan Huayi, Cai Ruxin, Li Yan*, Jia Honghua, Chen Kequan, Yan Ming, Ouyang Pingkai. Bioconversion of stevioside to rebaudioside E using glycosyltransferase UGTSL2. Applied Biochemistry and Biotechnology, 2021, 193: 637–649

5. Qi Xuelian, Shao Junlan, Cheng Yinchu, He Xiaoying, Li Yan*, Jia Honghua*, Yan Ming. Biocatalytic synthesis of 2-O-α-D-glucopyranosyl-L-ascorbic acid using an extracellular expressed α-glucosidase from Oryza sativa. Biotechnology Journal, 2021, 16(11): e2100199

6. Chen Liangliang, Cai Ruxin, Weng Jingyuan, Li Yan*, Jia Honghua, Chen Kequan, Yan Ming, Ouyang Pingkai. Production of rebaudioside D from stevioside using a UGTSL2 Asn358Phe mutant in a multi-enzyme system. Microbial Biotechnology, 2020, 13, 974–983

7. Sun Ping, Cai Ruxin, Chen Liangliang, Li Yan*, Jia Honghua, Yan Ming, Chen Kequan. Natural product glycosylation: Biocatalytic synthesis of quercetin-3,4′-O-diglucoside. Applied Biochemistry and Biotechnology, 2020, 190: 464–474

8. Li Yan*, Li Zheng, He Xiaoying, Chen Liangliang, Cheng Yinchu, Jia Honghua*, Yan Ming, Chen Kequan. Characterisation of a Thermobacillus sucrose phosphorylase and its utility in enzymatic synthesis of 2-O-alpha-D-glucopyranosyl-L-ascorbic acid. Journal of Biotechnology, 2019, 305: 27–34

9. Weng Jingyuan, Chen Liangliang, Cheng Yinchu, Li Yan*, Jia Honghua*, Zhou Hua, Wei Ping. Expression, characterization, and site-directed mutagenesis of UDP-glycosyltransferase UGT88A1 from Arabidopsis thaliana. Bioengineered, 2019, 4: 142-149.

10. Chen Liangliang, Sun Ping, Zhou Fangfang, Li Yan*, Chen Kequan, Jia Honghua, Yan Ming, Gong Dachun, Ouyang Pingkai. Synthesis of rebaudioside D, using glycosyltransferase UGTSL2 and in situ UDP-glucose regeneration. Food Chemistry, 2018, 259: 286–291

11. Cai Ruxin, Chen Caihong, Li Yan*, Sun Kaiyan, Zhou Fangfang, Chen Kequan, Jia Honghua. Improved soluble bacterial expression and properties of the recombinant flavonoid glucosyltransferase UGT73G1 from Allium cepa. Journal of Biotechnology, 2017, 255: 9–15

12. Chen Liangliang, Sun Ping, Li Yan*, Yan Ming, Xu Lin, Chen Kequan, Ouyang Pingkai. A fusion protein strategy for soluble expression of Stevia glycosyltransferase UGT76G1 in Escherichia coli. 3 Biotech, 2017, 7(6):356

13. Li Yan*, Li Yangyang, Wang Yu, Chen Liangliang, Yan Ming, Chen Kequan, Xu Lin, Ouyang Pingkai. Production of rebaudioside A from stevioside catalyzed by the engineered Saccharomyces cerevisiae. Applied Biochemistry & Biotechnology, 2016, 178(8): 1586–1598

14. Wang, Yu; Chen Liangliang, Li Yan*, Li Yangyang, Yan Ming, Chen Kequan, Hao Ning, Xu Lin. Efficient enzymatic production of rebaudioside A from stevioside. Bioscience Biotechnology & Biochemistry, 2015, 80(1): 67–73